Small hairpin RNA (shRNA) is a powerful tool for inhibiting gene expression. One limitation has been that this technique has been used primarily to target a single gene. This protocol expands upon ...

Murine leukemia virus (MLV)-based vectors are still the most frequently used gene delivery vehicles, employed in almost 40% of approved clinical protocols worldwide. 1,2 Despite their frequent use as ...

The baculovirus expression vector system (BEVS) provides a versatile platform for the expression of individual recombinant proteins as well as multimeric protein complexes, virus-like particles, ...

A universal vector (IL-60 and auxiliary constructs), expressing or silencing genes in every plant tested to date, is described. Plants that have been successfully manipulated by the IL-60 system ...

Recombinant protein expression is an essential pillar of biomedical research. Efforts from enzyme discovery to protein structure-function study to development of drugs and biological therapeutics all ...