Colorectal cancer is the second most common cause of cancer-related deaths in the United States, with an estimated 130 200 new cases and 56 300 deaths occurring in the year 2000 (American Cancer ...

Large-scale genomic analysis has changed the way we approach basic biology at the molecular level. Complementary DNA (cDNA) microarrays, as well as other high-throughput expression assays, have been ...

The Nottingham Prognostic Index (NPI) applied to 467 patients (pts) with a long-term follow-up that were treated in a single institution No significant financial relationships to disclose. This is an ...

Serial analysis of gene expression (SAGE) uses mRNA from a sample to create complementary DNA (cDNA) fragments for amplification and sequencing using high-throughput sequencing technology. Credit: ...

Processing cDNA microarray images is a crucial step in gene expression analysis, since any errors in early stages affect subsequent steps, leading to possibly erroneous biological conclusions. When ...

Yes, many are attracted to next-generation sequencing, but loyalty to chip-based phenotyping has its rewards, particularly in screens of large sample sets. The microarray, the legacy technology for ...

In each type of cell, like a muscle cell or a skin cell, different genes are expressed (turned on) or silenced (turned off). If the cells that are turned on mutate, they could—depending on what role ...

The main steps involved and limitations of microarray analysis will be encountered. Two stages can be distinguished in microarray experiments: a pre-microarray experiment phase (tissue handling) and ...

No significant financial relationships to disclose. This is an ASCO Meeting Abstract from the 2004 ASCO Annual Meeting. This abstract does not include a full text component.

Many cases of hereditary breast cancer are due to mutations in either the BRCA1 or the BRCA2 gene. The histopathological changes in these cancers are often characteristic of the mutant gene. We ...